Auteurs : Roger Wumba, Menotti Jean, Longo-Mbenza Benjamin, MandinaMadone, Kintoki Fabien, Zanga Josué, Sala Jean, Kendjo Eric, Guillo-Olczyk AC, and ThellierMarc
Hindawi Publishing Corporation Journal of Parasitology Research
Introduction
It is established that Enterocytozoon bieneusi (E. bieneusi) is the most commonly characterized microsporidia species among human beings. Microsporidia, obligate intracellular parasites, lack eukaryotic ribosomal features and peroxisomes
[1]. Their spores do penetrate and infect eukaryotic cells in various invertebrate and vertebrate organisms.
The literature reports epidemiology, causes, diagnosis, and digestive disorders related to microsporidiosis among HIVpatients[2–7].
In Kinshasa region, The capital city of The Democratic Republic of Congo (DRC), we detected E. bieneusi infection in HIV patients using only light microscopy and Fungi Fluor [8] as well as conventional polymerase chain reaction (PCR) method [9]. We could confirm the sensitivity of the diagnosis of E. bieneusi infection by a real-time PCR assay in comparison with traditional methods [10, 11].
E. bieneusi genotypes were also identified by PCRrestriction fragment length polymorphism (RFLP) analysis [12, 13].
Therefore, the objective of this study was to determine the prevalence and the genotypes of E. bieneusi in stool specimens among HIV patients by developing a rapid and efficient real-time PCR and PCR-RFLP approach.
Conclusion
The prevalence of E. bieneusi is emerging.We used a sensitive, specific, rapid, and efficient approach for typing E. bieneusi obtained from stool specimens by real-time PCR and PCRRFLP assays. Genotype I E. bieneusi is more prevalent among HIV-patients from Europe than the genotype I–genotype IV E. bieneusi estimated 1 in HIV-infected patients from the present study in Kinshasa, Democratic Republic of Congo.