Kabedi Bajani MJ1, Kayembe Ntumba JM2, Kayembe Kalambayi P3, Kashongwe Munogolo Z2, Lunguya Metila O1, Mujangi Kadima B1, Bisuta Fueza S2, Kabengele Obel B2,
Mbaya Kalumba P1, Taba Kalulu M4, Muyembe Tamfum JJ1

1Department of Microbiology, 2Department of Medicine Internal, 3School of Public Health,
Faculty of Medicine, University of Kinshasa, Democratic Republic of the Congo.
4Department of Chemistry Organic,
Faculty of Sciences, University of Kinshasa, Democratic Republic of the Congo.

ABSTRACT

Background : Multidrug-resistant tuberculosis (MDR-TB) is a real public health threat globally. Its early diagnosis remains an important means of reducing morbidity and mortality. The Ziehl-Neelsen staining, commonly used in tuberculosis detection and treatment centers, cannot be used to diagnose MDR-TB.
Objective : The objective of this study was to evaluate the performance of antibiogram slide in the detection of resistance to Rifampicin and to second-line molecules.
Methods : Prospective analysis of sputum smear from tuberculosis patients in therapeutic failure or relapse situations, comparing the performance of the antibiogram slide technique with respect to the gold standard the proportion method on LJ medium and molecular method of Gen-Xpert® MTB / RIF in the detection of resistance to antituberculosis drugs.
Results : After analysis, resistance to Rifampicin was observed in 47.3% of samples (142) using slide antibiogram test, 47% (141) with Gen-Xpert® MTB / RIF and 46.3 % (139) by the proportional method. The rate of MDR-TB was 39% and 9% pre XDR-TB. The sensitivity and specificity of the antibiogram slide was 97.9 and 100% with a positive predictive value of 100%. In contrast to Gen-Xpert® MTB / RIF, it was 97.87% and 99.83% and the positive predictive value was 99.28%.
Conclusion : Antibiogram slide showed non-inferiority to LJ and Gen-Xpert® in detecting resistance to Rifampicin and / or second-line molecules (Ofloxacin and Kanamycin) after 10 days incubation. Its dissemination with a view of its validation deserves to be considered in a low-cost area.
Key Words : Performance; Antibiogram on Slide; Multidrug-Resistant Tuberculosis; Kinshasa.

*Correspondence to:

Doctor Marie José Kabedi Bajani,
Department of Microbiology/University clinics of Kinshasa,
Faculty of Medicine/University of Kinshasa (DRC),
Democratic Republic of the Congo.
Email: bedye2001@yahoo.fr

Int J Med Res Prof.2017; 3(3); 348-52.

lien : www.ijmrp.com